EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.230 | 4-hydroxy-2(1H)-quinolone is produced by Escherichia coli coexpressing PqsA and PqsD | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.230 | 0.018 | - |
malonyl-[acyl-carrier protein] | pH 7.5, 37°C | Pseudomonas aeruginosa | |
2.3.1.230 | 0.035 | - |
2-aminobenzoyl-CoA | pH 7.5, 37°C | Pseudomonas aeruginosa | |
2.3.1.230 | 0.104 | - |
malonyl-CoA | pH 7.5, 37°C | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.230 | malonyl-CoA + 2-aminobenzoyl-CoA | Pseudomonas aeruginosa | the enzyme is involved in the biosynthesis of 2,4-dihydroxyquinoline. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is a highly interactive organism secreting a variety of chemical signals. One intercellular signaling system unique to Pseudomonas and Burkholderia species is the production of hydrophobic quinolines. These quinoline derivatives are 4-hydroxy-2-heptylquinoline, 3,4-dihydroxy-2-heptylquinoline, and 2,4-dihydroxyquinoline | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.230 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.230 | - |
Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.230 | 0.22 | - |
pH 7.5, 37°C | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.230 | malonyl-CoA + 2-aminobenzoyl-CoA | the enzyme is involved in the biosynthesis of 2,4-dihydroxyquinoline. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is a highly interactive organism secreting a variety of chemical signals. One intercellular signaling system unique to Pseudomonas and Burkholderia species is the production of hydrophobic quinolines. These quinoline derivatives are 4-hydroxy-2-heptylquinoline, 3,4-dihydroxy-2-heptylquinoline, and 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
2.3.1.230 | malonyl-CoA + 2-aminobenzoyl-CoA | first, the 2-aminobenzoyl moiety is transferred to the active-site Cys of PqsD to form a 2-aminobenzoyl-PqsD intermediate, which then condenses with either malonyl-CoA or malonyl-acyl carrier protein to produce 3-(2-aminophenyl)-3-oxopropanoyl-CoA. This short-lived intermediate undergoes an intramolecular rearrangement to form 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
2.3.1.230 | malonyl-[acyl-carrier protein] + 2-aminobenzoyl-CoA | first, the 2-aminobenzoyl moiety is transferred to the active-site Cys of PqsD to form a 2-aminobenzoyl-PqsD intermediate, which then condenses with either malonyl-CoA or malonyl-acyl carrier protein to produce 3-(2-aminophenyl)-3-oxopropanoyl-CoA. This short-lived intermediate undergoes an intramolecular rearrangement to form 2,4-dihydroxyquinoline | Pseudomonas aeruginosa | CoA + acyl-carrier protein + 4-hydroxy-2(1H)-quinolone + CO2 | i.e. 2,4-dihydroxyquinoline | ? | |
2.3.1.230 | additional information | no activity with malonic acid or 2-hydroxybenzoate | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.230 | 2,4-dihydroxyquinoline synthase | - |
Pseudomonas aeruginosa |
2.3.1.230 | PqsD | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.230 | 37 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.230 | 7.5 | - |
assay at | Pseudomonas aeruginosa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.230 | physiological function | synthesis of 2,4-dihydroxyquinoline, an extracellular metabolite produced by Pseudomonas aeruginosa and secreted into growth medium in stationary phase. The enzyme is essential for Pseudomonas quinolone signal synthesis | Pseudomonas aeruginosa |